Every horse or pony which
is admitted to the Laminitis Clinic has urine and blood samples collected and
submitted to the following analyses in order to try and identify any signs of
From one venepuncture samples
are collected into plain, heparin, oxalate/fluoride and EDTA transport tubes and
a urine sample is collected into a plain bottle.
From these samples we perform
the following analyses;
Haematology; full analysis
including platelet count and differential WBC count
Serum biochemistry for;
Alkaline phosphatase, intestinal alkaline phosphatase, AST, Gamma GT, Bile acids,
GLDH, LDH, Bilirubin, Total protein, Globulin, Albumin, Protein electrophoresis,
Urea, Creatinine, Glucose, Non esterified fatty acids, CPK, Sodium, Potassium,
Calcium, Magnesium, Chloride, Phosphate.
Urine; Creatinine, Sodium,
Potassium, Calcium, Magnesium, Chloride, Phosphate, pH, SG, blood, glucose, protein,
ketone, bilirubin, colour, sediment.
In addition should there
be any clinical signs indicating endocrine abnormalities, such as INSULIN RESISTANCE
OR CUSHING'S DISEASE we also collect samples to measure glucose, insulin and
ALL THESE SAMPLES SHOULD
BE TAKEN FIRST THING IN THE MORNING, BEFORE FEEDING, AND THE ANIMAL SHOULD IDEALLY
NOT BE RECEIVING ANY DRUGS. Your veterinary surgeon will arrange suitable transport
kits from a reputable laboratory and ensure samples arrive in good condition.
These are screening tests,
further testing may be necessary. For example an intravenous glucose tolerance
test is the gold standard for the identification of insulin resistance (metabolic
If endogenous ACTH is also
elevated, particularly in more than one sample and in combination with clinical
signs of Cushing's Disease then our chosen protocol is a combination of two herbal
products available from Equi Life Ltd.